Monoclonal antibodies were first produced in 1975 by Kohler and Milstein. They described how immunized mice spleen B cells fused with cancerous myeloma cell cells to create hybridoma cells which are both immortal and secrete an antibody of interest.
Although they are highly reproducible and specific, mouse monoclonal antibody delivery is not without limitations. They can also be sensitive to antigen heterogeneity or experimental conditions changes. You can know more about the advantages of rabbit monoclonal antibodies via Boster Bio.
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In recent years, rabbit monoclonal antibody has emerged as a key subset of the research antibody market. In-vitro diagnostics and ongoing clinical trials for therapeutic uses have led to the use of rabbit monoclonal antibodies for research in life sciences.
Scientists are recognizing the unique characteristics of these antibodies, which include a wider reactivity with animal tissue than mouse-derived antibodies and a stronger, diverse immune response.
Although the product range and depth have not yet been matched by traditional mouse monoclonal antibodies catalogs, scientific communities continue to demand more reliable and accurate reagents.
Recombinant rabbit monoclonal antibodies are similar in consistency to traditional mouse monoclonal antibodies while offering an animal-origin-free option.
Rabbits are immunized and peripheral blood mononuclear cells (PBMCs) are extracted and tested instead of hybridisms. The DNA is sub-cloned to a single clone and screened again after being cloned as a library and screened again. The resultant DNA is utilized to make animal-free formulations through cell culture.